1) Fragestellung

• Zonulin is discussed as a marker of increased intestinal permeability ...
• and is frequently associated in clinical terminology with the concept of “leaky gut.”
• In a general medical practice, fecal zonulin levels were measured in patients with chronic health problems...
• ...and were subsequently reassessed during follow-up after a NANA10-based intervention combined with optimization of vitamin D status.

Keywords

• Zonulin; leaky gut; intestinal permeability; NANA10®; vitamin D; stool sample; follow-up; observational study; real-world data; EIA (Enzyme Immuno Assay)

2) Methode

• The aim of this retrospective analysis was to summarize the course of fecal zonulin levels in a shared dataset of 128 patients undergoing a NANA10-based intervention.
• The analysis combines four previously separate assessments:
• the primary before-and-after effect,
• durability across multiple measurement time points,
• observation of a relapse during an interruption of intake,
• and a Kaplan–Meier-like presentation of the normalization of pathologically elevated zonulin levels.
• A total of 128 patients from a general medical practice were evaluated.
• The mean age was 53.7 years, with a range from 2 to 79 years.
• Zonulin was measured in stool samples.
• The intervention consisted of optimization of vitamin D levels to 50–100 ng/ml ...
• ... and intake of NANA10, a combination of ten natural dietary supplement components.
• Further information is available at www.NANA10.de.
• Laboratory analysis was performed using an EIA test system from Immundiagnostik AG.
• Non-exactly quantified extreme values were coded conservatively for the analysis;
• for example, values reported as “>800” were entered as exactly 800 ng/ml,
• and values reported as “<15” or “<5” were entered as exactly 15 or exactly 5 ng/ml, respectively.
• For the primary analysis, paired values at time point 1 (Z1) and Z2 were evaluated in n=128 patients.
• Additional available longitudinal values up to Z7 were also considered.
• In all patients, a vitamin D level of 50–100 ng/ml was additionally maintained or, where necessary, achieved.
• The instrument used to model vitamin D dosing was www.VitaminDSimulator.de.

3) Ergebnisse

• The median fecal zonulin level decreased from 179.5 ng/ml at Z1 to 82.7 ng/ml at Z2.
• The Wilcoxon signed-rank test for paired data showed a highly significant decrease from Z1 to Z2 (n=128; p=2.48 ×10^-17).
• The median of the absolute differences was 85.0 ng/ml, and the median relative change corresponded to a decrease of 56.5%.
• In the sign test, 109 of 128 individuals showed a decrease (p=7.78 × 10^-17).
• The proportion of patients above the reference limit of 131 ng/ml decreased from 59.38% at Z1 to 21.09% at Z2.
• The exact McNemar test confirmed this reduction with p=5.91 × 10^-12.
• Among 48 complete cases with Z1–Z3 values, the Friedman test showed a significant multi-time-point difference (chi-square=45.33; p=1.43 × 10^-10).
• A log-linear time model using all available measurements yielded a multiplicative monthly factor of 0.95485,
• corresponding to an estimated decrease of 4.52% per month (p=4.11 × 10^-6).
• In a Kaplan–Meier-like assessment, the proportion of pathologically elevated values above 131 ng/ml
• remained at approximately 60% after 5 months,
• approximately 30% after 10 months,
• approximately 10% after 15 months,
• and approximately 3% from about 20 months onward.

4) Folgerungen

• In this retrospective practice-based follow-up analysis, ...
• ...a clear and statistically highly significant reduction in fecal zonulin levels was observed
• ...under a NANA10-based intervention combined with vitamin D optimization.
• The effect was visible not only at the first follow-up but also in longer-term longitudinal assessments.
• Due to the uncontrolled observational design, the combined intervention, ...
• and the absence of randomization, a causal attribution to NANA10 alone is not possible.
• Nevertheless, the findings are relevant as hypothesis-generating real-world data and justify prospective controlled studies.

Quellenangaben

Publikation auf RESEARCHGATE
 

1.
Dr. med. Raimund von Helden. TEST-ERGEBNIS: NANA10 Erfolg geht nicht verloren.
VitaminDservice.de, February 8, 2026.
https://www.vitaminDservice.de/node/8480

2.
Dr. med. Raimund von Helden. NaNa10 ist gut wirksam gegen den Leaky-Gut-Parameter
Zonulin. (n=128). VitaminDservice.de, February 8, 2026.
https://www.vitaminDservice.de/node/8479

3.
Dr. med. Raimund von Helden. Leaky-Gut-Rückfall mit Fortsetzung von NaNa10 gestoppt.
VitaminDservice.de, February 17, 2026.
https://www.vitaminDservice.de/node/8504

4.
Dr. med. Raimund von Helden. Das Leaky-Gut- oder Zonulin-Syndrom verschwindet unter
NaNa10 nach 1,5 Jahren. VitaminDservice.de, February 17, 2026.
https://www.vitaminDservice.de/node/8503

5.
Immundiagnostik AG. Information on the zonulin test system and reference range, as reported
in the underlying partial texts.
https://www.immundiagnostik.com

6.
Labor Bochum. Laboratory analysis, as reported in the underlying partial texts.
https://medlab-bochum.de

7.
VitaminDservice.de. Normalwert-Diskussion Zonulin.
https://www.vitamindservice.de/NORMALWERT-ZONULIN

8.
VitaminDservice.de. NaNa10 Anbieter / Bezug.
https://www.vitamindservice.de/nana10/ANBIETER

9.
VitaminDservice.de. Zonulin in der publizierten Forschung / Kompass Zonulin.
https://www.vitamindservice.de/KOMPASS-ZONULIN

Kurzbeschreibung (deutsch):

• In einer retrospektiven, praxisbasierten Follow-up-Analyse wurden 128 Patientinnen und Patienten mit chronischen Gesundheitsproblemen und einer zonulinbezogenen klinischen Fragestellung ausgewertet.
• Unter einer NANA10-basierten Intervention in Kombination mit einer Vitamin-D-Optimierung sank der Median des fäkalen Zonulinspiegels von 179,5 ng/ml auf 82,7 ng/ml.
• Der Anteil oberhalb der Referenzgrenze von 131 ng/ml verringerte sich von 59,38 % auf 21,09 %.
• Gepaarte nichtparametrische Tests, der McNemar-Test, der Friedman-Test sowie ein log-lineares Zeitmodell zeigten konsistent hochsignifikante Abnahmen.
• Longitudinalanalysen sprechen für eine weitgehend nachhaltige Reduktion;
• eine Kaplan-Meier-ähnliche Darstellung weist auf eine substanzielle Normalisierung pathologischer Werte über einen Zeitraum von etwa 18 bis 20 Monaten hin.
• Aufgrund des retrospektiven, unkontrollierten Designs sind die Ergebnisse hypothesengenerierend und sollten in prospektiven kontrollierten Studien überprüft werden.